IRCT20210919052515N3 IRCT 2022-02-13 Oroumia University of Medical Sciences Assessment of the amniotic membrane effect in anal fissure wound Assessment of the amniotic membrane effect on healing of anal fissure wound after surgery 2022-02-20 anticipated 110 Complete https://irct.ir/trial/61556 interventional Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Treatment, Randomization description: Patients will be divided into intervention and control groups using block randomization based on generated numbers by random allocation software. Thus, in this software, first the number of groups and the total number of the sample size will be entered, and then in the block section, the Block randomization method will be selected. Patients will be allocated in intervention or control groups based on generated numbers. 3 Fissure wound. Intervention 1: Intervention group: in addition to standard sphincterotomy surgery, two Cm2 of amniotic membrane prepared from the stem cell center of the Tehran University will be sutured to the margin of the anal fissure wound. Intervention 2: In the control group, only standard sphincterotomy surgery will be performed. No - There is not a plan to make this available Justification or reason for not sharing IPD is Confidentiality of patient information