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IRCT20171009036662N1 IRCT 2026-01-07 Iran National Science Foundation The effect of two resistance training models on hormones, muscle performance, and delayed onset muscle soreness in untrained men The effect of two resistance training models, rest-pause and superset, on hormones related to hypertrophy and muscle metabolism, delayed onset muscle soreness, and collagen breakdown in untrained men 2026-01-21 anticipated 24 Complete https://irct.ir/trial/88370 interventional Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Education/Guidance, Randomization description: Participants will be randomly assigned to either the first intervention group or the second intervention group using a random number table. To ensure allocation concealment, sealed, opaque, and sequentially numbered envelopes will be used so that neither the researcher nor the participants are aware of the assigned group until each individual is enrolled in the study. Group allocation will be performed with a 1:1 ratio. N/A The effect of two resistance training models, rest-pause and superset, on hormones related to hypertrophy and muscle metabolism, delayed onset muscle soreness, and collagen breakdown in untrained men. Intervention 1: Intervention group: The resistance training program will be performed using a superset method at an intensity of 75% of 1-RM. Participants will perform the training session with 10 minutes of warm-up prior to the exercise and 10 minutes of cool-down following the exercise. A total of eight exercises will be included: bench press, reverse-grip lat pulldown, leg extension, leg curl, good morning, Bulgarian squat, biceps curl, and cable triceps extension.The execution tempo will be controlled so that the concentric phase lasts 2 seconds and the eccentric phase lasts 3 seconds. In this training system, two exercises will be performed consecutively as a superset, with each exercise consisting of four sets, and 16, 16, 20, and 24 repetitions per set for both exercises. A 90-second rest interval within each set between the paired exercises and a 2-minute rest period after completion of each superset pair will be provided. Intervention 2: Intervention group: The rest–pause resistance training protocol will be performed using free weights, targeting large muscle groups at an intensity of 85% of one-repetition maximum (1-RM). Participants will complete 10 minutes of warm-up before and 10 minutes of cool-down after the training session.A total of eight exercises will be performed, including bench press, reverse-grip lat pulldown, leg extension, leg curl, good morning, Bulgarian squat, biceps curl, and triceps pushdown using a cable machine.The execution tempo will be controlled so that the concentric contraction lasts 2 seconds and the eccentric phase lasts 3 seconds.In this resistance training protocol, each exercise will be performed once (18 total repetitions), divided into three rest–pause sets (4–6–8 repetitions). A 10-second intra-set rest will be provided between segments, and a 2-minute rest period will be allocated between exercises. Yes - There is a plan to make this available What will be shared: All data on measured variables can be shared after de-identifying the participants. When: Data Access begins from 2026 To whom: Only for researchers affiliated with academic and scientific institutions Conditions: Scientific use in improvement and comparison with other studies is permitted. Where to obtain: Email address for correspondence with the researcher to obtain necessary information: Mehrabanij@guilan.ac.ir How to obtain: The applicant must send the reason for using the documents via email, and after being reviewed by the researcher, a response will be provided as soon as possible Mehrabanij@guilan.ac.ir Comments:
public Pegah Movahedpour
5th Kilometer of Persian Gulf Highway, Rasht, Guilan Province, Iran
Rasht Iran (Islamic Republic of) 4199613776 +98 13 3369 0274 pmovahedpour@webmail.ac.ir The University of Guilan scientific Javad Mehrabani
5th Kilometer of Persian Gulf Highway, Rasht, Guilan Province, Iran
Rasht Iran (Islamic Republic of) 4199613776 +98 13 3369 0685 mehrabanij@guilan.ac.ir The University of Guilan Iran (Islamic Republic of) man not trained in 4-6 months men aged 20 to 27 years, inclusive 20 years 27 years Male skeletal, muscular, cardiovascular, or hormonal disorders women and trained individuals smokers Other Other Intervention group: The resistance training program will be performed using a superset method at an intensity of 75% of 1-RM. Participants will perform the training session with 10 minutes of warm-up prior to the exercise and 10 minutes of cool-down following the exercise. A total of eight exercises will be included: bench press, reverse-grip lat pulldown, leg extension, leg curl, good morning, Bulgarian squat, biceps curl, and cable triceps extension.The execution tempo will be controlled so that the concentric phase lasts 2 seconds and the eccentric phase lasts 3 seconds. In this training system, two exercises will be performed consecutively as a superset, with each exercise consisting of four sets, and 16, 16, 20, and 24 repetitions per set for both exercises. A 90-second rest interval within each set between the paired exercises and a 2-minute rest period after completion of each superset pair will be provided. Intervention group: The rest–pause resistance training protocol will be performed using free weights, targeting large muscle groups at an intensity of 85% of one-repetition maximum (1-RM). Participants will complete 10 minutes of warm-up before and 10 minutes of cool-down after the training session.A total of eight exercises will be performed, including bench press, reverse-grip lat pulldown, leg extension, leg curl, good morning, Bulgarian squat, biceps curl, and triceps pushdown using a cable machine.The execution tempo will be controlled so that the concentric contraction lasts 2 seconds and the eccentric phase lasts 3 seconds.In this resistance training protocol, each exercise will be performed once (18 total repetitions), divided into three rest–pause sets (4–6–8 repetitions). A 10-second intra-set rest will be provided between segments, and a 2-minute rest period will be allocated between exercises. Growth Hormone (GH). Timepoint: Before the exercise and immediately after the exercise. Method of measurement: Blood samples from the brachial vein in a seated position, following standard hygienic procedures. Insulin-like Growth Factor (IGF-1). Timepoint: Before the exercise and immediately after the exercise. Method of measurement: Blood samples from the brachial vein in a seated position, following standard hygienic procedures. Cortisol. Timepoint: Before the exercise and immediately after the exercise. Method of measurement: Blood samples from the brachial vein in a seated position, following standard hygienic procedures. Testosterone. Timepoint: Before the exercise and immediately after the exercise. Method of measurement: Blood samples from the brachial vein in a seated position, following standard hygienic procedures. Lactate. Timepoint: Before the exercise and immediately after the exercise. Method of measurement: Blood samples from the brachial vein in a seated position, following standard hygienic procedures. Creatine Kinase (CK). Timepoint: Before the activity and 24, 48, and 72 hours after completing the activity. Method of measurement: Blood samples from the brachial vein in a seated position, following standard hygienic procedures. Lactate Dehydrogenase (LDH). Timepoint: Before the activity and 24, 48, and 72 hours after completing the activity. Method of measurement: Blood samples from the brachial vein in a seated position, following standard hygienic procedures. Hydroxy lysine. Timepoint: Before the activity and 24, 48, and 72 hours after completing the activity. Method of measurement: Blood samples from the brachial vein in a seated position, following standard hygienic procedures. Hydroxy proline. Timepoint: Before the activity and 24, 48, and 72 hours after completing the activity. Method of measurement: Blood samples from the brachial vein in a seated position, following standard hygienic procedures. Rating of Perceived Exertion(RPE). Timepoint: During the activity, immediately after the activity, and 24, 48, and 72 hours after completing the activity. Method of measurement: Using the Borg scale. Muscle soreness. Timepoint: During the activity, immediately after the activity, and 24, 48, and 72 hours after completing the activity. Method of measurement: Using the Visual Analogue Scale(VAS). Range of Motion(ROM). Timepoint: Before the activity and 24, 48, and 72 hours after completing the activity. Method of measurement: Using a protractor. Exercise Performance. Timepoint: Before the activity and 24, 48, and 72 hours after completing the activity. Method of measurement: Using the leg press and chest press machines. Iran National Science Foundation Approved 2025-11-03 Ethics committee in biomedical research of Guilan University Guilan University, 5th kilometer of Persian Gulf Highway, Rasht, Guilan Province, Iran Rasht Guilan Iran (Islamic Republic of)