In this study, therapeutic effects of an anti-gravity locomotor training on central nervous system structure and function, neuromuscular properties and gait in children with cerebral palsy will be studied. 100 children with cerebral palsy will be referred to neural engineering and rehabilitation center of Noorafshar hospital from rehabilitation and medical centers. 4-14 years old hemiplegic patients with spasticity in lower extremities and also with the ability of following commands who are diagnosed by pediatric neurologists will be randomly separated to study and control groups. The study group will be trained on the anti-gravity treadmill and the control group will receive over-ground walking training by an expert occupational therapist. Each group will receive up to 1 hour of training for 3 days/week for 2 months. The anti-gravity treadmill will facilitate treadmill walking with reduced lower extremity weight bearing (up to %80), which can help patients walk voluntary and actively on the treadmill. It is expected that this systematic, intensive training result in structural changes (neuroplasticity) in central nervous system, so that the nerve conduction will be facilitated and improved in neural pathways. It is expected that this systematic, intensive training result in structural changes (neuroplasticity) in central nervous system, so that the nerve conduction will be facilitated and improved in neural pathways. As a result of this neuroplasticity, the treatment effects will be long lasting and improve the children’s quality of life. In addition to evaluating the clinical changes, brain structural changes, activity level of the descending neural pathways including corticospinal tracts, neuromuscular properties, balance, and the lower limbs movement ability of the children will be quantified.