The effect of a combined training session on ABCA5 and ApoB gene expression and white blood cell lipid profile in middle-aged men after cardiac bypass surgery
The effect of eight weeks of resistance training on the expression of ABCG5 gene, APOB plasma levels And middle-aged men 's lipid profile after coronary artery bypass grafting
Design
The present research design will be quasi-experimental and laboratory research. The statistical population of the present study is 60 middle-aged male patients who underwent coronary artery bypass grafting at least 2 and at most 4 months ago and will be referred to Javad Al-A'meh Heart Hospital in Mashhad. The statistical sample will be selected voluntarily from the statistical population according to the conditions of entering the research. Among them, 30 men who underwent bypass surgery, with an age range of 65-50 years and by available sampling method were selected and divided into two experimental groups (15 people) and control group (15 people). be. These exercises will be performed in rehabilitation phase 3 and patients will perform exercise exercises in this phase for the first time.
Settings and conduct
The quasi-experimental study will be completed with a pre-test-post-test design in three intervention groups in Javad Al-A'meh Hospital under the supervision of a physician for eight weeks.
Participants/Inclusion and exclusion criteria
Inclusion criteria (Cognitive health, visual and auditory, no blood pressure higher than 160 mm Hg and diastolic higher than 100 mm Hg. Do not use the same drugs, do not use assistive devices such as walkers and age range 50 to 65 years who underwent coronary artery bypass grafting Criteria for non-admission were lack of permission from a cardiologist and lack of inclusion criteria
Intervention groups
The intervention in the present study will be eight weeks of endurance and resistance training.
The effect of a combined training session on ABCA5 and ApoB gene expression and white blood cell lipid profile in middle-aged men after cardiac bypass surgery
Public title
The effect of combined rehabilitation exercises in patients with coronary artery bypass grafting
Purpose
Prevention
Inclusion/Exclusion criteria
Inclusion criteria:
Age range 50-60 years
Willingness to participate in research
Cognitive, visual and auditory health
No systolic blood pressure higher than 160 mmHg and diastolic blood pressure higher than 100 mmHg
No acute and advanced diseases that prevent the exercises considered in this study.
Exclusion criteria:
Existence of symptoms and heart failure
Taking over-the-counter medications
Inability to perform sports activities
Age
From 50 years old to 60 years old
Gender
Male
Phase
3
Groups that have been masked
No information
Sample size
Target sample size:
60
Actual sample size reached:
30
Randomization (investigator's opinion)
Randomized
Randomization description
The samples will be determined by random blocking method with 4 blocks using the table of random numbers of Software Allocation Random software. Blocking and allocation sequencing for concealment will be done by the person not involved in the research.
Blinding (investigator's opinion)
Not blinded
Blinding description
Placebo
Not used
Assignment
Parallel
Other design features
In fact, this study was performed to investigate the effect of endurance and resistance training on the physiological status of patients with coronary artery bypass grafting.
Secondary Ids
empty
Ethics committees
1
Ethics committee
Name of ethics committee
Ethics Committee of Islamic Azad University, Neyshabour Branch
Street address
RESEARCH Ave, Islamic Azad University Complex Neyshabur Branch
City
neyshabur
Province
Razavi Khorasan
Postal code
9319797139
Approval date
2020-09-24, 1399/07/03
Ethics committee reference number
IR.IAU.NEYSHABUR.REC.1400.001
Health conditions studied
1
Description of health condition studied
Chronic ischaemic heart disease, unspecified
ICD-10 code
I25.7
ICD-10 code description
Atherosclerosis of coronary artery bypass graft(s) and coronary artery of transplanted heart with angina pectoris
Primary outcomes
1
Description
ABCG5 gene expression
Timepoint
24 hours before the start of the protocol and 48 hours after the end of the last training protocol session
Method of measurement
REAL TIME-PCR method
2
Description
APOB plasma levels
Timepoint
24 hours before the start of the protocol and 48 hours after the end of the last training protocol session
Method of measurement
ELISA laboratory method
3
Description
LDL plasma levels
Timepoint
24 hours before the start of the protocol and 48 hours after the end of the last training protocol session
Method of measurement
ELISA laboratory method
Secondary outcomes
empty
Intervention groups
1
Description
Intervention group: Aerobic-resistance training group. After taking blood samples and functional tests (5-second unassisted sit-up test (TUG), 3-meter back and forth (BBS) and cardiorespiratory function (6-minute walk)), participants are asked Start the training session with an aerobic program in three parts: walking on a treadmill (20 to 30 minutes), cycling (10 to 12 minutes) and manual ergometer (10 minutes) with an intensity of 60% of maximum heart rate. Then, by performing a resistance training program in three consecutive sets with 8 to 15 repetitions, the training session ends. The intensity of resistance training can be changed by using different colors (yellow to pink) of Traband. Then, at the end of the training period, blood is taken again and the functional test is measured again.
Category
Prevention
2
Description
Intervention group: Resistance-aerobic training group. After taking blood samples and functional tests (5-second unassisted sit-up test (TUG), 3-meter back and forth (BBS) and cardiorespiratory function (6-minute walk)) from participants Is asked to Perform resistance training program in three consecutive sets with 8 to 15 repetitions. The intensity of resistance training can be changed by using different colors (yellow to pink) of Traband. Then the training session is completed with an aerobic program in three parts that included walking on a treadmill (20 to 30 minutes), pedaling on a bicycle (10 to 12 minutes) and hand ergometer (10 minutes) with an intensity of 60% of maximum heart rate. Also, at the end of the training period, blood is taken again and the functional test is measured again.
Category
Prevention
3
Description
Control group: did not have any sports activities and supplementation and illegal drugs during the research protocol
Category
Prevention
Recruitment centers
1
Recruitment center
Name of recruitment center
Javadalameh Specialized Heart Hospital, Mashhad
Full name of responsible person
Dr. Amir Rashid Lamir
Street address
Vakil abad Ave. Honarestan Blvd.
City
Mashhad
Province
Razavi Khorasan
Postal code
9178613111
Phone
+98 51 3881 6010
Fax
+98 51 3883 8103
Email
info@jhosp.org
Web page address
http://www.jhosp.org
Sponsors / Funding sources
1
Sponsor
Name of organization / entity
Islamic Azad University
Full name of responsible person
Dr. Ahmad Zendeh Del
Street address
No 38. Hashemiyeh14.
City
Mashhad
Province
Razavi Khorasan
Postal code
9173613111
Phone
+98 51 3880 3000
Email
m_akbari64@hotmail.com
Web page address
http://www.jhosp.org
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Islamic Azad University
Proportion provided by this source
100
Public or private sector
Private
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic
Person responsible for general inquiries
Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Dr. Amir Rashid Lamir
Position
Professor
Latest degree
Ph.D.
Other areas of specialty/work
Physiology
Street address
Vakil Abad Ave. Park Square
City
Mashhad
Province
Razavi Khorasan
Postal code
9173613111
Phone
+98 51 3880 3000
Email
m_akbari64@hotmail.com
Web page address
http://info@jhosp.org
Person responsible for scientific inquiries
Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Mohsen Akbari
Position
Consultant
Latest degree
Ph.D.
Other areas of specialty/work
Physiology
Street address
No38. Vakil Abad Ave. Hashemiyeh Blvd.
City
Mashhad
Province
Razavi Khorasan
Postal code
917861311
Phone
+98 51 3880 3000
Email
m_akbary64@hotmail.com
Web page address
http://info@jhosp.org
Person responsible for updating data
Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Mohsen Akbari
Position
Consultant
Latest degree
Ph.D.
Other areas of specialty/work
Physiology
Street address
No38. Vakil Abad Ave. Hashemiyeh Blvd. Hashemiyeh 14.
City
Mashhad
Province
Razavi Khorasan
Postal code
9178613111
Phone
+98 51 3880 6000
Email
m_akbary64@hotmail.com
Web page address
http://info@jhosp.org
Sharing plan
Deidentified Individual Participant Data Set (IPD)
No - There is not a plan to make this available
Justification/reason for indecision/not sharing IPD
We apologize for publishing the data because of the ethical commitment it has made to patients and the Patient Rehabilitation Center.
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Yes - There is a plan to make this available
Data Dictionary
Yes - There is a plan to make this available
Title and more details about the data/document
The data file will be provided as a spss file
When the data will become available and for how long
From 1400 to 1402
To whom data/document is available
Researchers and researchers and academic and scientific institutions
Under which criteria data/document could be used
There is data usage and analysis for researchers
From where data/document is obtainable
To use the data, refer to the email m_akbari64@hotmail.com
What processes are involved for a request to access data/document
Email the person in charge of correspondence
Comments
Except for ethical criteria, the rest of the data will be made available to researchers