Protocol summary

Study aim
Investigating the effect of 6 weeks of high-intensity interval training in the form of exercise snacks on cardiometabolic and renal markers in overweight/obese adults
Design
A randomized, single‑blinded, parallel‑group, controlled clinical trial on 60 patients. For randomization, opaque sealed envelopes will be used.
Settings and conduct
Study setting: Payame Noor University, Alborz Province, Karaj Branch; study population: overweight or obese adults; type of blinding: laboratory technicians will not be aware of the group allocation when assessing blood samples.
Participants/Inclusion and exclusion criteria
Inclusion Criteria: Participants were eligible for inclusion if they were sedentary and physically inactive, overweight or obese adults of both sexes with a body mass index of 25 kg/m² or greater, aged 35 to 55 years, in general good health or free from any serious illness or unstable condition requiring medical supervision over diet or physical activity, and without any physical limitations or restrictions. Exclusion Criteria: pregnancy, being currently adhering to a structured weight-loss program or using weight-loss medications, failed to obtain physician approval for study participation.
Intervention groups
Intervention Group 1: HIIT2; performing 2 bouts of high-intensity interval training per day with intervals of 1–4 hours apart, 3 days per week. Intervention Group 2: HIIT6; performing 6 bouts of high-intensity interval training per day with intervals of 1–4 hours apart, 3 days per week. Control Group: C; no training.
Main outcome variables
Cardiometabolic index; visceral adiposity index; lipid accumulation product; uric acid; urea

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20241008063290N3
Registration date: 2026-08-01, 1405/05/10
Registration timing: prospective

Last update: 2026-08-01, 1405/05/10
Update count: 0
Registration date
2026-08-01, 1405/05/10
Registrant information
Name
hamid rajabi
Name of organization / entity
Kharazmi university
Country
Iran (Islamic Republic of)
Phone
+98 21 8832 9220
Email address
hrajabi@hotmail.com
Recruitment status
recruiting
Funding source
Expected recruitment start date
2026-08-06, 1405/05/15
Expected recruitment end date
2026-10-07, 1405/07/15
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effect of 6-week high intensity interval training using an exercise snack training on cardiometabolic and renal indicators in overweight/obese adults
Public title
The effect of an exercise training period on cardiometabolic and renal markers in overweight/obese adults
Purpose
Supportive
Inclusion/Exclusion criteria
Inclusion criteria:
Sedentary and physically inactive adults Overweight or obese individuals Body mass index (BMI) ≥ 25 kg/m² Both men and women Aged 35–55 years In generally good health Free from any serious illness or unstable medical condition requiring physician supervision over diet or physical activity No physical limitations or restrictions affecting exercise participation
Exclusion criteria:
Having acute cardiovascular disease Following specific dietary regimes With weight instability (+_5 kg) in the past 3 months Having any serious medical condition that precludes long-term participation in physical activity Pregnancy Currently adhering to a structured weight-loss program Using weight-loss medications Failure to obtain physician approval for study participation
Age
From 35 years old to 55 years old
Gender
Both
Phase
N/A
Groups that have been masked
  • Outcome assessor
Sample size
Target sample size: 60
Randomization (investigator's opinion)
Randomized
Randomization description
Simple randomization will be used for participant allocation. To ensure concealment, a series of opaque, sealed envelopes will be prepared. For this purpose, envelopes labeled with the name of either the exercise intervention group or the control group will be created, corresponding to the total number of subjects. After thoroughly mixing (shuffling) the envelopes, each participant will select one, and their group assignment will be recorded accordingly
Blinding (investigator's opinion)
Single blinded
Blinding description
Clinical laboratory technicians, unaware of the group allocation, will assess the blood parameters.
Placebo
Not used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Research Ethics Committees of Kharazmi University
Street address
Kharazmi University, Shahid Keshvari Stadium, Shahid Hesari Street, Madar Square
City
Tehran
Province
Tehran
Postal code
14911-15719
Approval date
2026-05-26, 1405/03/05
Ethics committee reference number
IR.KHU.REC.1405.029

Health conditions studied

1

Description of health condition studied
Adults who are overweight or obese
ICD-10 code
E66
ICD-10 code description
Overweight and obesity

Primary outcomes

1

Description
Cardiometabolic index
Timepoint
24–48 hours before and after the intervention
Method of measurement
By means of the formula

2

Description
Visceral adiposity index
Timepoint
24–48 hours before and after the intervention
Method of measurement
By means of the formula

3

Description
Lipid accumulation product
Timepoint
24–48 hours before and after the intervention
Method of measurement
By means of the formula

4

Description
Triglyceride
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

5

Description
High-density lipoprotein
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

6

Description
Low-density lipoprotein
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

7

Description
Total cholesterol
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

8

Description
The ratio of high-density lipoprotein to low-density lipoprotein
Timepoint
24–48 hours before and after the intervention
Method of measurement
By means of the formula

9

Description
Thyroid-Stimulating Hormone
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

10

Description
Thyroxine
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

11

Description
Bilirubin
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

12

Description
Urea
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

13

Description
Uric acid
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

14

Description
Creatinine
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

15

Description
estimated Glomerular Filtration Rate
Timepoint
24–48 hours before and after the intervention
Method of measurement
By ELISA or enzymatic laboratory method

Secondary outcomes

1

Description
Systolic blood pressure
Timepoint
24–48 hours before and after the intervention
Method of measurement
Using a German Beurer device

2

Description
Diastolic blood pressure
Timepoint
24–48 hours before and after the intervention
Method of measurement
Using a German Beurer device

3

Description
Resting heart rate
Timepoint
24–48 hours before and after the intervention
Method of measurement
Using a German Beurer device

4

Description
Mean arterial pressure
Timepoint
24–48 hours before and after the intervention
Method of measurement
Using the formula

5

Description
Body Mass Index
Timepoint
24–48 hours before and after the intervention
Method of measurement
Using the formula

6

Description
Waist-to-hip circumference ratio
Timepoint
24–48 hours before and after the intervention
Method of measurement
Using the formula

7

Description
Waist-to-height ratio
Timepoint
24–48 hours before and after the intervention
Method of measurement
Using the formula

8

Description
Body weight
Timepoint
24–48 hours before and after the intervention
Method of measurement
Scale

9

Description
height
Timepoint
24–48 hours before the intervention
Method of measurement
Wall-mounted height measure

10

Description
Wall squat test
Timepoint
24–48 hours before the intervention
Method of measurement
By recording the duration

11

Description
30-Second Chair Stand Test
Timepoint
24–48 hours before the intervention
Method of measurement
By counting the repetitions

12

Description
Quality of Life
Timepoint
24–48 hours before the intervention
Method of measurement
Questionnaire

Intervention groups

1

Description
Control group: Control group participants are to remain inactive throughout the study and are advised to follow their usual daily routines.
Category
Other

2

Description
Intervention group 1: Two-Bout High-Intensity Interval Training: The training protocol includes 2 bouts of exercise, each containing 4 sets of 2-minute body-weight HIIT exercises at 85% of maximal heart rate (HRmax). Each set is interspersed with 1 minute and 30 seconds of active recovery (at 65% HRmax) between successive sets.
Category
Rehabilitation

3

Description
Intervention group 2: Six-Bout High-Intensity Interval Training: The training protocol includes 6 exercise bouts, each containing 2 sets of 80-second body-weight HIIT exercises at 85% of maximal heart rate (HRmax). Each set is interspersed with 1 minute of active recovery (at 65% HRmax) between successive sets.
Category
Rehabilitation

Recruitment centers

1

Recruitment center
Name of recruitment center
Payame Noor University, Alborz Province, Karaj Branch
Full name of responsible person
Dr. Farokh kia
Street address
Shahid Kamali Dehghan Boulevard, Shahid Moazen Boulevard
City
Karaj
Province
Alborz
Postal code
3149968143
Phone
+98 26 3574 1055
Email
alborz.pnu@ac.ir
Web page address
http://alborz.pnu.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Iran National Science Foundation
Full name of responsible person
Ali Mohammad Soltani
Street address
No. 33, Fifth Street, above Jalal Al Ahmad intersection, North Kargar Street.
City
Tehran
Province
Tehran
Postal code
۱۴۳۹۶۳۴۶۶۵
Phone
+98 21 8216 1000
Fax
+98 21 8800 3981
Email
rms@insf.org
Web page address
https://insf.org/fa
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Iran National Science Foundation
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Other

Person responsible for general inquiries

Contact
Name of organization / entity
Kharazmi University
Full name of responsible person
Hamid Rajabi
Position
professor
Latest degree
Ph.D.
Other areas of specialty/work
exercise physiology
Street address
Faculty of Sports Sciences of Kharazmi University, Shahid Keshvari Stadium, Shahid Hesari Street, Mader Square, Tehran
City
Tehran
Province
Tehran
Postal code
1954812345
Phone
0098 21 88329220-3
Fax
Email
hrajabi@khu.ac.ir
Web page address
https://pess.khu.ac.ir/

Person responsible for scientific inquiries

Contact
Name of organization / entity
Kharazmi University
Full name of responsible person
Hamid Rajabi
Position
Professor
Latest degree
Ph.D.
Other areas of specialty/work
Exercise physiology
Street address
Faculty of Sports Sciences of Kharazmi University, Shahid Keshvari Stadium, Shahid Hesari Street, Mader Square, Tehran
City
Tehran
Province
Tehran
Postal code
1954812345
Phone
0098 21 88329220-3
Fax
Email
hrajabi@khu.ac.ir
Web page address
https://pess.khu.ac.ir/

Person responsible for updating data

Contact
Name of organization / entity
Kharazmi University
Full name of responsible person
Hamid Rajabi
Position
professor
Latest degree
Ph.D.
Other areas of specialty/work
exercise physiology
Street address
Faculty of Sports Sciences of Kharazmi University, Shahid Keshvari Stadium, Shahid Hesari Street, Mader Square, Tehran
City
Tehran
Province
Tehran
Postal code
1954812345
Phone
0098 21 88329220-3
Fax
Email
hrajabi@khu.ac.ir
Web page address
https://pess.khu.ac.ir/

Sharing plan

Deidentified Individual Participant Data Set (IPD)
No - There is not a plan to make this available
Justification/reason for indecision/not sharing IPD
There is no further information
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Yes - There is a plan to make this available
Data Dictionary
Not applicable
Title and more details about the data/document
NO
When the data will become available and for how long
Data is not published
To whom data/document is available
Data is not published
Under which criteria data/document could be used
Data is not published
From where data/document is obtainable
Data is not published
What processes are involved for a request to access data/document
Data is not published
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